The Ability of Streptomyces spp. Isolated from Iranian Soil to Solubilize Rock Phosphate

Nazila Biglari, Hasnuri Mat Hassan, Javid Amini

Abstract


Many agricultural soils are deficient in plant available phosphate and hence not able to sustain optimal crop productivity. The problem is due to the ability of many soils to fix phosphate in a wide range of soil pH and ecological conditions. There is a need to search for more environmental friendly alternatives to improve soil fertility and crop production in phosphate deficient soil. The aim of this study was to isolate, screen, and characterize phosphate solubilizing actinomycetes found in different types of soil with varied pH from various sites in Iran. Phosphate solubilizing ability of the actinomycetes was evaluated both on modified Pikovskaya's (PVK) agar and into broth media containing Christmas Island Rock Phosphate (CIRP). The abilities of each isolate to solubilize phosphorus was evaluated from day 1 to day 14 after inoculation. Streptomyces spp. were identified morphologically under scanning electron microscope (SEM). About 31% (22/70) isolates of actinomycetes were found to have the ability to solubilize (CIRP). Isolates IA15 and IA31 showed high solubilizing index (SI) on agar medium whereas isolates IA61, IA59, IA38, IA35, and IA31 were determined to have high CIRP solubilizing ability in broth medium. Isolates IA11, IA31, IA10, and IA61 had high pH decrease in broth medium after 14 days of inoculation. A gradual decrease in pH was observed over a 14 day period of incubation, suggesting a slow release of phosphate from CIRP. The mechanism of solubilization was related to pH decrease in broth medium. In general, majority of phosphate solubilizing actinomycetes revealed superior ability to solubilize CIRP.

 

Key words: rock phosphate; solubilizing phosphate; Streptomyces spp;


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References


ACHAL, V., SAVANT, V. & REDDY, M. S. 2007. Phosphate solubilization by a wild type strain and UV-induced mutants of Aspergillus tubingensis. Soil Biology and Biochemistry, 39, 695-699.

AL-HALBOUNI, D., TRABER, J., LYKO, S., WINTGENS, T., MELIN, T., TACKE, D., JANOT, A., DOTT, W. & HOLLENDER, J. 2008. Correlation of EPS content in activated sludge at different sludge retention times with membrane fouling phenomena. Water Research, 42, 1475-1488.

ASUMING-BREMPONG, S. & AFERI, N. 2014. Isolation of phosphate solubilizing bacteria from tropical soil. Global advanced research journal of agricultural science, 3, 008-015.

BAN, J.-G., KIM, H.-B., LEE, M.-J., ANBU, P. & KIM, E.-S. 2014. Identification of a vitamin D3-specific hydroxylase genes through actinomycetes genome mining. Journal of industrial microbiology and biotechnology, 41, 265-273.

BAREA, J., TORO, M., OROZCO, M., CAMPOS, E. & AZCÓN, R. 2002. The application of isotopic (32P and 15N) dilution techniques to evaluate the interactive effect of phosphate-solubilizing rhizobacteria, mycorrhizal fungi and Rhizobium to improve the agronomic efficiency of rock phosphate for legume crops. Nutrient Cycling in Agroecosystems, 63, 35-42.

BRÄNNVALL, E., NILSSON, M., SJÖBLOM, R., SKOGLUND, N. & KUMPIENE, J. 2014. Effect of residue combinations on plant uptake of nutrients and potentially toxic elements. Journal of environmental management, 132, 287-295.

BUSARAKAM, K., BULL, A. T., GIRARD, G., LABEDA, D. P., VAN WEZEL, G. P. & GOODFELLOW, M. 2014. Streptomyces leeuwenhoekii sp. nov., the producer of chaxalactins and chaxamycins, forms a distinct branch in Streptomyces gene trees. Antonie van Leeuwenhoek, 105, 849-861.

CASTANHEIRA, N., DOURADO, A. C., ALVES, P. I. L., CORTÉS-PALLERO, A. M., DELGADO-RODRÍGUEZ, A. I., PRAZERES, Â., BORGES, N., SÁNCHEZ, C., CRESPO, M. T. B. & FARELEIRA, P. 2014. Annual ryegrass-associated bacteria with potential for plant growth promotion. Microbiological Research.

COUTINHO, F. P., FELIX, W. P. & YANO-MELO, A. M. 2012. Solubilization of phosphates in vitro by Aspergillus spp. and Penicillium spp. Ecological Engineering, 42, 85-89.

DASTAGER, S. G. & DAMARE, S. 2013. Marine actinobacteria showing phosphate-solubilizing efficiency in Chorao Island, Goa, India. Current microbiology, 66, 421-427.

GANGWAR, M., RANI, S. & SHARMA, N. 2012. Investigating endophytic actinomycetes diversity from Rice for plant growth promoting and antifungal activity. International Journal of Advanced Life Sciences (IJALS), 1.

GUPTA, N., SAHOO, D. & BASAK, U. C. 2010. Evaluation of in vitro solubilization potential of phosphate solubilizing Streptomyces isolated from phyllosphere of Heritiera fomes (mangrove). African Journal of Microbiology Research, 4, 136-142.

HAMDALI, H., BOUIZGARNE, B., HAFIDI, M., LEBRIHI, A., VIROLLE, M. J. & OUHDOUCH, Y. 2008. Screening for rock phosphate solubilizing Actinomycetes from Moroccan phosphate mines. Applied Soil Ecology, 38, 12-19.

KAPRI, A. & TEWARI, L. 2010. Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp. Brazilian Journal of Microbiology, 41, 787-795.

KARAGÖZ, K., ATEŞ, F., KARAGÖZ, H., KOTAN, R. & ÇAKMAKÇı, R. 2012. Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils. European Journal of Soil Biology, 50, 144-150.

KAVIYARASI, K., KANIMOZHI, K., MADHANRAJ, P., PANNEERSELVAM, A. & AMBIKAPATHY, V. 2011. Isolation, Identification and Molecular Characterization of Phosphate Solubilizing Actinomycetes Isolated From The Coastal Region of Manora, Thanjavur (Dt.). Asian J. Pharm. Tech, 1, 119-122.

KHAN, M. S., ZAIDI, A. & MUSARRAT, J. 2014. Phosphate Solubilizing Microorganisms: Principles and Application of Microphos Technology, Springer.

MEHTA, P., WALIA, A., KULSHRESTHA, S., CHAUHAN, A. & SHIRKOT, C. K. 2014. Efficiency of plant growth promoting P solubilizing Bacillus circulans CB7 for enhancement of tomato growth under net house conditions. Journal of basic microbiology.

NESME, T., COLOMB, B., HINSINGER, P. & WATSON, C. A. 2014. Soil phosphorus management in organic cropping systems: from current practices to avenues for a more efficient use of P resources. Organic farming, prototype for sustainable agricultures. Springer.

NGOSONG, C., GABRIEL, E. & RUESS, L. 2014. Collembola grazing on arbuscular mycorrhiza fungi modulates nutrient allocation in plants. Pedobiologia.

OGBO, F. C. 2010. Conversion of cassava wastes for biofertilizer production using phosphate solubilizing fungi. Bioresource Technology, 101, 4120-4124.

RAHMAN, M. M., SALLEH, M. A. M., RASHID, U., AHSAN, A., HOSSAIN, M. M. & RA, C. S. 2014. Production of slow release crystal fertilizer from wastewaters through struvite crystallization. Arabian journal of chemistry, 7, 139-155.

RAJPUT, A., PANHWAR, Q., NAHER, U., RAJPUT, S., HOSSAIN, E. & SHAMSHUDDIN, J. 2014. Influence of incubation period, temperature and different phosphate levels on phosphate adsorption in soil. American journal of agricultural and biological sciences, 9, 251.

RODRı́GUEZ, H. & FRAGA, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances, 17, 319-339.

SADEGHI, A., KARIMI, E., DAHAJI, P., JAVID, M., DALVAND, Y. & ASKARI, H. 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology, 28, 1503-1509.

SAHU, M. K., SIVAKUMAR, K., THANGARADJOU, T. & KANNAN, L. 2007. Phosphate solubilizing actinomycetes in the estuarine environment: An inventory. Journal of Environmental Biology, 28, 795-798.

SALCEDO, L. D. P., PRIETO, C. & CORREA, M. F. 2014. Screening phosphate solubilizing actinobacteria isolated from the rhizosphere of wild plants from the eastern Cordillera of the Colombian Andes. African Journal of Microbiology Research, 8, 734-742.

SHARMA, P., DAS, R., KALITA, M. C. & THAKUR, D. 2014. Investigation of extracellular antifungal proteinaceous compound produced by Streptomyces sp. 5K10. African journal of microbiology research, 8, 986-993.

STANECK, J. L. & ROBERTS, G. D. 1974. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Applied microbiology, 28, 226-231.

SUJATHA, N. & AMMANI, K. 2014. Phosphate solubilization by the isolates of fluorescent pseudomonads. International journal of advances in pharmacy, biology and chemistry 3.

TALLAPRAGADA, P. & SESHACHALA, U. 2012. Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India. Turk. J. Biol, 36, 25-35.

TSAO, H.-W., MICHINAKA, A., YEN, H.-K., GIGLIO, S., HOBSON, P., MONIS, P. & LIN, T.-F. 2014. Monitoring of geosmin producing Anabaena circinalis using quantitative PCR. Water research, 49, 416-425.

VIANI, R. A., RODRIGUES, R. R., DAWSON, T. E., LAMBERS, H. & OLIVEIRA, R. S. 2014. Soil pH accounts for differences in species distribution and leaf nutrient concentrations of Brazilian woodland savannah and seasonally dry forest species. Perspectives in plant ecology, evolution and systematics, 16, 64-74.

WHITELAW, M. 1999. Growth promotion of plants inoculated with phosphate-solubilizing fungi. Advances in Agronomy, 69, 99-151.


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