Adhesion of Streptococcus mutans to Zirconia, Titanium Alloy and some other Restorative Materials: “An in-vitro Study”

Ezzatollah Jalalian, Shahbaz Naser Mostofi, Elnaz Shafiee, Amin Nourizadeh, Reihaneh Aghajani Nargesi, Sarah Ayremlou

Abstract


Introduction: Bacterial adhesion on restorative materials may lead to gingival inflammation and secondary caries.

Objectives: The aim of this in vitro study was to evaluate the adhesion of streptococcus mutans to zirconia, Feldespatic porcelain, titanium alloy and Indirect composite resin In-vitro. The effect of surface roughness on bacterial adhesion was also studied.

Materials and Methods: 10 specimens (5mm diameter, 1mm thickness) of each material, Zirconia, Indirect composite resin, Titanium alloy and Feldespatic porcelain were fabricated. Enamel was used as reference. Specimens were covered with artificial saliva and bacterial suspension (109 CFU/mL). Bacterial adhesion was determined using scanning electron microscope and culturing the specimens in blood agar. Data were analyzed with One way ANOVA followed by Tukey post hoc test for roughness and Kruskal-wallis test for adhesion values.

Results: The highest bacterial adhesion was recorded for composite specimens and the lowest was seen in Ziconia group (p<0.5). The mean value of adhesion for zirconia, feldespatic peocelain, Titanium alloy and indirect composite were 28±6.32, 40.80±8.40, 75±4.47 and 386±13.75, respectively. The differences between zirconia and titanium alloy and also zirconia and indirect composite and porcelain and indirect composite were statistically significant (p<0.5).

Conclusion: Zirconia showed the lowest bacterial adhesion in comparison to other tested materials and Enamel. The difference between zirconia and titanium alloy and also zirconia and indirect composite was statistically significant (p<0.5). No correlation was found between surface roughness and bacterial adhesion.

 

Keywords: Bacterial adhesions; Streptococcus mutans; Dental caries


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